Investigators at SSI and Radboudumc previously evaluated the 10C fragment of Pfs48/45 as a fusion to the N-terminal region (R0) of the asexual blood stage antigen Glutamate-rich Protein (GLURP). The chimeric protein R0.10C was produced in Lactococcus lactis and induced robust antibody titers in rats and specific antibodies showed strong transmission blocking activity against multiple strains of Plasmodium falciparum in the standard membrane feeding assay (SMFA). To improve the yield of correctly folded Pfs48/45, a novel chimera consisting of the 6C fragment (which includes 6 cysteine residues rather than 10 in the 10C fragment), similarly produced in L. lactis, was also shown to elicit high titer transmission blocking antibodies. Results on the immunogenicity of these fragments are expected in the first quarter of 2016.
Roeffen W, Theisen M, van de Vegte-Bolmer M, van Gemert G, Arens T, et al.
Transmission-blocking activity of antibodies to Plasmodium falciparum GLURP.10C chimeric protein formulated in different adjuvants.
Malaria Journal. 2015; 14(443).