While A. stephensi mosquitoes are primarily used in standard membrane feeding assay (SMFA) testing of vaccine concepts, A. gambiae mosquitoes are a common malaria vector in sub-Saharan Africa. This article, published in Parasites & Vectors, compares SMFA estimates of transmission-reducing activity for A. stephensi and A. gambiae mosquitoes using human serum and monoclonal pre-fertilization (anti-Pfs48/45) and post-fertilization (anti-Pfs25) antibodies known to effectively inhibit sporogony. The findings support the use of A. stephensi rather than A. gambiae mosquitoes in SMFA target prioritization.