6 Apr 2017
Author(s): 
Taylor BJ, Lanke K, Banman SL, Morlais I, Morin MJ, et al.

Currently, there are few tools available to effectively monitor submicroscopic infections, particularly gametocytes, in low-resource or high-transmission settings. This article, published in The American Journal of Tropical Medicine and Hygiene, describes the development and testing of the Reverse Transcriptase Polymerase Chain Reaction Assay (RT-PCR), which detects Plasmodium falciparum asexual and sexual stage RNA targets directly from blood samples in a one-step test. The authors conclude that due to the assay's high sensitivity for gametocyte-specific activity and its ease of use with portable real-time PCR instruments, this assay would be a potentially effective malaria transmission monitoring tool in field settings.