This article, published in The American Journal of Tropical Medicine and Hygiene, reports findings of a study conducted in Mali which identified and optimized parameters that lead to successful mosquito feeding outcomes in both direct skin feeds (DSFs) and direct membrane feeding assays (DFMA). These assays are critical tools in detecting parasite transmission in a standardized manner, and thus, developing transmission-blocking malaria vaccines (TBV). The study found that younger mosquitoes were significantly associated with higher feeding, survival, and infection rates. Other variables such as membrane type and location of the mosquito bite did not significantly affect infection outcome. The article makes specific recommendations for optimal practices for DSFs and DFMAs for further TBV development.
5 Sep 2017